Application
A. Western blot analysis on HepG2 cell lysate using anti-CK-19 RabMAb (Cat. #AJ1224b), dilution 1:1,000,000Application
B. Immunofluorescent staining of HeLa cells using anti-CK-19 RabMAb (Cat. #AJ1224b).Application
2a. Immunohistochemical staining of paraffin-embedded human kidney carcinoma using anti-CK-19 RabMAb (Cat. #AJ1224b).Application
2b. Negative IHC staining of paraffin-embedded hepatocellular carcinoma using anti-Keratin-19 RabMAb (Cat. #AJ1224b).| Product Name | Cytokeratin-19 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KRT19, Keratin, type I cytoskeletal 19, Keratin-19 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1580Y |
| Host Species | Rabbit |
| Tested Applications | WBIFIHC |
| WB:1:1000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | KRT19 Keratin type I cytoskeletal 19 Keratin-19 |
|---|---|
| Molecular Weight(MW) | 44106 Da |
| Function | Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle |
| Tissue Specificity | Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin |
| Entrez Gene | 3880 |
|---|
Application
A. Western blot analysis on HepG2 cell lysate using anti-CK-19 RabMAb (Cat. #AJ1224b), dilution 1:1,000,000
Application
B. Immunofluorescent staining of HeLa cells using anti-CK-19 RabMAb (Cat. #AJ1224b).
Application
2a. Immunohistochemical staining of paraffin-embedded human kidney carcinoma using anti-CK-19 RabMAb (Cat. #AJ1224b).
Application
2b. Negative IHC staining of paraffin-embedded hepatocellular carcinoma using anti-Keratin-19 RabMAb (Cat. #AJ1224b).| Application Notes | WB:1:1000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
