Application
DAG1 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the DAG1 antibody detected the DAG1 protein (arrow).Application
DAG1 Antibody (C-term) (AP14101b)immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DAG1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | DAG1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DAG1; Dystroglycan; Dystrophin-associated glycoprotein 1; Alpha-dystroglycan; Beta-dystroglycan |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | DAG1 Dystroglycan Dystrophin-associated glycoprotein 1 Alpha-dystroglycan Beta-dystroglycan |
|---|---|
| Molecular Weight(MW) | 97441 Da |
| Function | The dystroglycan complex is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization Beta-dystroglycan is a transmembrane protein that plays important roles in connecting the extracellular matrix to the cytoskeleton. Acts as a cell adhesion receptor in both muscle and non-muscle tissues. Receptor for both DMD and UTRN and, through these interactions, scaffolds axin to the cytoskeleton. Also functions in cell adhesion-mediated signaling and implicated in cell polarity |
| Tissue Specificity | Expressed in a variety of fetal and adult tissues. In epidermal tissue, located to the basement membrane Also expressed in keratinocytes and fibroblasts |
| Cellular Localization | Alpha-dystroglycan: Secreted, extracellular space |
| Entrez Gene | 1605 |
|---|---|
| Nucleotide Accession | NP_001171107.1 NP_001171111.1 NP_004384.4 |
Application
DAG1 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the DAG1 antibody detected the DAG1 protein (arrow).
Application
DAG1 Antibody (C-term) (AP14101b)immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DAG1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
