Application
A. Western blot analysis on rat spleen lysate using anti-DAPK2 RabMAb (Cat. #AJ1235a), dilution 1:2000.Application
B. Immunohistochemical staining of paraffin-embedded human liver carcinoma using anti-DAPK2 RabMAb (Cat. #AJ1235a).| Product Name | DAPK2 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DAPK2, Death-associated protein kinase 2, DAP-kinase-related protein 1 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1633Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | DAPK2 Death-associated protein kinase 2 DAP-kinase-related protein 1 |
|---|---|
| Molecular Weight(MW) | 42898 Da |
| Function | Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Acts as a mediator of anoikis and a suppressor of beta-catenin-dependent anchorage-independent growth of malignant epithelial cells. May play a role in granulocytic maturation |
| Tissue Specificity | Isoform 2 is expressed in embryonic stem cells (at protein level). Isoform 1 is ubiquitously expressed in all tissue types examined with high levels in heart, lung and skeletal muscle. It is expressed abundantly in cells differentiated toward granulocytes and low in undifferentiated, normal and leukemic hematopoietic cells and monocytes/macrophages |
| Cellular Localization | Cytoplasm. Cytoplasmic vesicle, autophagosome lumen |
| Entrez Gene | 23604 |
|---|
Application
A. Western blot analysis on rat spleen lysate using anti-DAPK2 RabMAb (Cat. #AJ1235a), dilution 1:2000.
Application
B. Immunohistochemical staining of paraffin-embedded human liver carcinoma using anti-DAPK2 RabMAb (Cat. #AJ1235a).| Application Notes | WB:1:1000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
