Application
A. Western blot analysis on HeLa cell lysate using anti-DAPK3 RabMAb (Cat. #AJ1236a).| Product Name | DAPK3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DAPK3, ZIPK, Death-associated protein kinase 3, ZIP-kinase |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR1636Y |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ZIPK |
|---|---|
| Alternative Names | DAPK3 ZIPK Death-associated protein kinase 3 ZIP-kinase |
| Molecular Weight(MW) | 52536 Da |
| Function | Serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, translation, actin cytoskeleton reorganization, cell motility, smooth muscle contraction, and mitosis, particularly cytokinesis. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase- dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles Regulates myosin phosphorylation in both smooth muscle and non- muscle cells. In smooth muscle, regulates myosin either directly by phosphorylating MYL12B and MYL9 or through inhibition of smooth muscle myosin phosphatase (SMPP1M) via phosphorylation of PPP1R12A, and the inhibition of SMPP1M functions to enhance muscle responsiveness to Ca(2+) and promote a contractile state. Enhances transcription from AR-responsive promoters in a hormone- and kinase-dependent manner. Phosphorylates STAT3 and enhances its transcriptional activity. Positively regulates the canonical Wnt/beta-catenin signaling through interaction with NLK and TCF7L2. Can disrupt the NLK-TCF7L2 complex thereby influencing the phosphorylation of TCF7L2 by NLK. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. Involved in the formation of promyelocytic leukemia protein nuclear body (PML-NB), one of many subnuclear domains in the eukaryotic cell nucleus, and which is involved in oncogenesis and viral infection. Phosphorylates RPL13A on 'Ser-77' upon interferon-gamma activation which is causing RPL13A release from the ribosome, its association with the GAIT complex and its subsequent involvement in transcript- selective translation inhibition |
| Tissue Specificity | Isoform 2 is expressed in the bladder smooth muscle |
| Cellular Localization | Nucleus. Cytoplasm. Nucleus, PML body. Chromosome, centromere. Cytoplasm, cytoskeleton, centrosome Note=The phosphorylated form is anchored in the cytoplasm and/or prevented from being shuttled to the nucleus, whereas nuclear translocation or retention is maximal when it is not phosphorylated. Phosphorylation at Thr-299 promotes cytoplasmic localization. Relocates to the cytoplasm on binding PAWR where the complex appears to interact with actin filaments. Localizes to promyelocytic leukemia protein nuclear bodies (PML-NBs) Associated: with the centrosomes throughout the mitotic cell cycle, with the centromeres from prophase to anaphase and with the contractile ring during cytokinesis |
| Entrez Gene | 1613 |
|---|
Application
A. Western blot analysis on HeLa cell lysate using anti-DAPK3 RabMAb (Cat. #AJ1236a).| Application Notes | WB:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
