Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with DARS antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of anti-DARS Pab in K562 cell line lysates (35ug/lane).DARS (arrow) was detected using the purified Pab (1:60 dilution).Application
Western blot analysis of DARS (arrow) using rabbit polyclonal DARS Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DARS gene.| Product Name | DARS Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DARS; Aspartate--tRNA ligase, cytoplasmic; Aspartyl-tRNA synthetase; Cell proliferation-inducing gene 40 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| IHC:1:10~50 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | DARS Aspartate--tRNA ligase cytoplasmic Aspartyl-tRNA synthetase Cell proliferation-inducing gene 40 protein |
|---|---|
| Molecular Weight(MW) | 57136 Da |
| Function | Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA |
| Tissue Specificity | This DARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 472~501 amino acids from the C-terminal region of human DARS. |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 1615 |
|---|
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with DARS antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of anti-DARS Pab in K562 cell line lysates (35ug/lane).DARS (arrow) was detected using the purified Pab (1:60 dilution).
Application
Western blot analysis of DARS (arrow) using rabbit polyclonal DARS Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DARS gene.| Application Notes | IHC:1:10~50 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
