Application
Western blot analysis of DBF4B Antibody (Center) in MDA-MB435 cell line lysates (35ug/lane). DBF4B (arrow) was detected using the purified Pab.Application
DBF4B Antibody (Center) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | DBF4B Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DBF4B; ASKL1; DRF1; Protein DBF4 homolog B; Activator of S phase kinase-like protein 1; Chiffon homolog B; Dbf4-related factor 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ASKL1 DRF1 |
|---|---|
| Alternative Names | DBF4B ASKL1 DRF1 Protein DBF4 homolog B Activator of S phase kinase-like protein 1 Chiffon homolog B Dbf4-related factor 1 |
| Molecular Weight(MW) | 67243 Da |
| Function | Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S and M phases The complex CDC7-DBF4B selectively phosphorylates MCM2 subunit at 'Ser-40' and then is involved in regulating the initiation of DNA replication during cell cycle |
| Tissue Specificity | Widely expressed. Highly expressed in testis. |
| Cellular Localization | Nucleus. Note=Predominantly found in soluble fraction but not in the chromatin-bound fraction |
| Entrez Gene | 80174 |
|---|
Application
Western blot analysis of DBF4B Antibody (Center) in MDA-MB435 cell line lysates (35ug/lane). DBF4B (arrow) was detected using the purified Pab.
Application
DBF4B Antibody (Center) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
