Application
DBH Antibody (N-term P42) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the DBH antibody detected the DBH protein (arrow).Application
DBH Antibody (N-term P42) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DBH Antibody (N-term P42) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of DBH Antibody (N-term P42)(Cat#AP11226a) with HepG2 cell followed by Alexa Fluor搴?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
Confocal immunofluorescent analysis of DBH Antibody (N-term P42)(Cat#AP11226a) with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | DBH Antibody (N-term P42) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DBH; Dopamine beta-hydroxylase; Dopamine beta-monooxygenase; Soluble dopamine beta-hydroxylase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | DBH Dopamine beta-hydroxylase Dopamine beta-monooxygenase Soluble dopamine beta-hydroxylase |
|---|---|
| Molecular Weight(MW) | 69065 Da |
| Function | Conversion of dopamine to noradrenaline. |
| Tissue Specificity | This DBH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 34-64 amino acids from the N-terminal region of human DBH. |
| Cellular Localization | Soluble dopamine beta-hydroxylase: Cytoplasmic vesicle, secretory vesicle lumen. Cytoplasmic vesicle, secretory vesicle, chromaffin granule lumen |
Application
DBH Antibody (N-term P42) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the DBH antibody detected the DBH protein (arrow).
Application
DBH Antibody (N-term P42) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DBH Antibody (N-term P42) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of DBH Antibody (N-term P42)(Cat#AP11226a) with HepG2 cell followed by Alexa Fluor搴?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
Confocal immunofluorescent analysis of DBH Antibody (N-term P42)(Cat#AP11226a) with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
