Application
Western blot analysis of DBT Antibody (Center) in Hela cell line lysates (35ug/lane). DBT (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded mouse kidney tissue reacted with DBT Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
DBT Antibody (Center) flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of DBT Antibody (Center)(Cat#AP6831c) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | DBT Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DBT; BCATE2; Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial; Branched-chain alpha-keto acid dehydrogenase complex component E2; Dihydrolipoamide acetyltransferase component of branched-chain alph |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFCIF |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BCATE2 |
|---|---|
| Alternative Names | DBT BCATE2 Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex mitochondrial Branched-chain alpha-keto acid dehydrogenase complex component E2 Dihydrolipoamide acetyltransferase component of branched-chain alph |
| Molecular Weight(MW) | 53487 Da |
| Function | The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3) |
| Tissue Specificity | This DBT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 240~270 amino acids from the Center region of human DBT. |
| Cellular Localization | Mitochondrion matrix. |
| Entrez Gene | 1629 |
|---|
Application
Western blot analysis of DBT Antibody (Center) in Hela cell line lysates (35ug/lane). DBT (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded mouse kidney tissue reacted with DBT Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
DBT Antibody (Center) flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of DBT Antibody (Center)(Cat#AP6831c) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
