Application
Western blot analysis of DCI Antibody (C-term) in mouse heart tissue(lane 1) and MCF-7(lane 2), HL-60(lane 3) cell line lysates (35ug/lane). DCI (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with DCI Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
DCI Antibody (C-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | DCI Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ECI1; DCI; Enoyl-CoA delta isomerase 1, mitochondrial; 3, 2-trans-enoyl-CoA isomerase; Delta(3), Delta(2)-enoyl-CoA isomerase; Dodecenoyl-CoA isomerase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | DCI |
|---|---|
| Alternative Names | ECI1 DCI Enoyl-CoA delta isomerase 1 mitochondrial 3 2-trans-enoyl-CoA isomerase Delta(3) Delta(2)-enoyl-CoA isomerase Dodecenoyl-CoA isomerase |
| Molecular Weight(MW) | 32816 Da |
| Function | Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species |
| Tissue Specificity | This DCI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 273~302 amino acids from the C-term region of human DCI. |
| Cellular Localization | Mitochondrion matrix. |
| Entrez Gene | 1632 |
|---|
Application
Western blot analysis of DCI Antibody (C-term) in mouse heart tissue(lane 1) and MCF-7(lane 2), HL-60(lane 3) cell line lysates (35ug/lane). DCI (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with DCI Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
DCI Antibody (C-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
