Application
Western blot analysis of DCL-1 Antibody (Center) in mouse liver tissue lysates (35ug/lane). DCL-1 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma with DCL-1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
DCL-1 Antibody (Center) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | DCL-1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD302; CLEC13A; DCL1; KIAA0022; CD302 antigen; C-type lectin BIMLEC; C-type lectin domain family 13 member A; Type I transmembrane C-type lectin receptor DCL-1; CD_antigen=CD302; Flags: Precursor |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CLEC13A DCL1 KIAA0022 |
|---|---|
| Alternative Names | CD302 CLEC13A DCL1 KIAA0022 CD302 antigen C-type lectin BIMLEC C-type lectin domain family 13 member A Type I transmembrane C-type lectin receptor DCL-1 CD_antigen=CD302 Flags: Precursor |
| Molecular Weight(MW) | 26183 Da |
| Function | Potential multifunctional C-type lectin receptor that may play roles in endocytosis and phagocytosis as well as in cell adhesion and migration |
| Tissue Specificity | Expressed at moderate levels in monocytes, myeloid blood dendritic cells and granulocytes and at low levels in plasmacytoid blood dendritic cells, monocyte-derived ma crophages and monocyte-derived dendritic cells, with no expression detected in T-lymphocytes, B-lymphocytes and natural killer cells (at protein level). Expressed widely in different tissues, with highest expression levels in liver, lung, peripheral blood leukocytes and spleen, and lowest levels in neuronal tissues, skeletal muscle and ovary. Isoform 2 and isoform 3 are expressed in malignant Hodgkin lymphoma cells called Hodgkin and Reed- Sternberg (HRS) cells |
| Cellular Localization | Membrane; Single-pass type I membrane protein (Potential). Cell projection, filopodium. Cytoplasm, cell cortex. Cell projection, microvillus. Note=Colocalizes with F- actin in filopodia, cellular cortex and microvilli of the apical cell surface |
| Entrez Gene | 9936 |
|---|
Application
Western blot analysis of DCL-1 Antibody (Center) in mouse liver tissue lysates (35ug/lane). DCL-1 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma with DCL-1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
DCL-1 Antibody (Center) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
