Application
Western blot analysis of DCP2 Antibody (Center) in HL-60 cell line lysates (35ug/lane). DCP2 (arrow) was detected using the purified Pab.Application
DCP2 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DCP2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
DCP2 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | DCP2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DCP2; NUDT20; m7GpppN-mRNA hydrolase; Nucleoside diphosphate-linked moiety X motif 20; mRNA-decapping enzyme 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NUDT20 |
|---|---|
| Alternative Names | DCP2 NUDT20 m7GpppN-mRNA hydrolase Nucleoside diphosphate-linked moiety X motif 20 mRNA-decapping enzyme 2 |
| Molecular Weight(MW) | 48457 Da |
| Function | Decapping metalloenzyme that catalyzes the cleavage of the cap structure on mRNAs. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Plays a role in replication-dependent histone mRNA degradation. Has higher activity towards mRNAs that lack a poly(A) tail. Has no activity towards a cap structure lacking a RNA moiety |
| Tissue Specificity | Expressed in brain and testis. Not detected in heart (at protein level) |
| Cellular Localization | Cytoplasm, P-body. Nucleus. Note=Predominantly cytoplasmic, in processing bodies (PB). A minor amount is nuclear |
| Entrez Gene | 167227 |
|---|
Application
Western blot analysis of DCP2 Antibody (Center) in HL-60 cell line lysates (35ug/lane). DCP2 (arrow) was detected using the purified Pab.
Application
DCP2 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DCP2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
DCP2 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
