Application
DCLRE1B Antibody (Center) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the DCLRE1B antibody detected DCLRE1B protein (arrow).Application
DCLRE1B Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DCLRE1B Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | DCLRE1B Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DCLRE1B; SNM1B; 5' exonuclease Apollo; DNA cross-link repair 1B protein; SNM1 homolog B |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SNM1B |
|---|---|
| Alternative Names | DCLRE1B SNM1B 5' exonuclease Apollo DNA cross-link repair 1B protein SNM1 homolog B |
| Molecular Weight(MW) | 60002 Da |
| Function | 5'-3' exonuclease that plays a central role in telomere maintenance and protection during S-phase. Participates in the protection of telomeres against non-homologous end-joining (NHEJ)- mediated repair, thereby ensuring that telomeres do not fuse Plays a key role in telomeric loop (T loop) formation by being recruited by TERF2 at the leading end telomeres and by processing leading-end telomeres immediately after their replication via its exonuclease activity: generates 3' single-stranded overhang at the leading end telomeres avoiding blunt leading-end telomeres that are vulnerable to end-joining reactions and expose the telomere end in a manner that activates the DNA repair pathways. Together with TERF2, required to protect telomeres from replicative damage during replication by controlling the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Also involved in response to DNA damage: plays a role in response to DNA interstrand cross-links (ICLs) by facilitating double-strand break formation. In case of spindle stress, involved in prophase checkpoint |
| Tissue Specificity | This DCLRE1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 215-244 amino acids from the Central region of human DCLRE1B. |
| Cellular Localization | Chromosome, telomere. Nucleus. Cytoplasm, cytoskeleton, centrosome. Note=Mainly localizes to telomeres, recruited via its interaction with TERF2. During mitosis, localizes to the centrosome |
| Entrez Gene | 64858 |
|---|---|
| Nucleotide Accession | NP_073747.1 |
Application
DCLRE1B Antibody (Center) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the DCLRE1B antibody detected DCLRE1B protein (arrow).
Application
DCLRE1B Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DCLRE1B Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
