Application
DCPS Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the DCPS antibody detected the DCPS protein (arrow).| Product Name | DCPS Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DCPS; DCS1; HINT5; m7GpppX diphosphatase; DCS-1; Hint-related 7meGMP-directed hydrolase; Histidine triad protein member 5; Scavenger mRNA-decapping enzyme DcpS |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | DCS1 HINT5 |
|---|---|
| Alternative Names | DCPS DCS1 HINT5 m7GpppX diphosphatase DCS-1 Hint-related 7meGMP-directed hydrolase Histidine triad protein member 5 Scavenger mRNA-decapping enzyme DcpS |
| Molecular Weight(MW) | 38609 Da |
| Function | Decapping scavenger enzyme that catalyzes the cleavage of a residual cap structure following the degradation of mRNAs by the 3'->5' exosome-mediated mRNA decay pathway. Hydrolyzes cap analog structures like 7-methylguanosine nucleoside triphosphate (m7GpppG) with up to 10 nucleotide substrates (small capped oligoribonucleotides) and specifically releases 5'-phosphorylated RNA fragments and 7-methylguanosine monophosphate (m7GMP). Cleaves cap analog structures like tri-methyl guanosine nucleoside triphosphate (m3(2,2,7)GpppG) with very poor efficiency. Does not hydrolyze unmethylated cap analog (GpppG) and shows no decapping activity on intact m7GpppG-capped mRNA molecules longer than 25 nucleotides. Does not hydrolyze 7-methylguanosine diphosphate (m7GDP) to m7GMP (PubMed:22985415). May also play a role in the 5'->3 mRNA decay pathway; m7GDP, the downstream product released by the 5'->3' mRNA mediated decapping activity, may be also converted by DCPS to m7GMP (PubMed:14523240). Binds to m7GpppG and strongly to m7GDP. Plays a role in first intron splicing of pre- mRNAs. Inhibits activation-induced cell death |
| Tissue Specificity | Detected in liver, brain, kidney, testis and prostate |
| Cellular Localization | Cytoplasm. Nucleus. Note=Predominantly localized in the nucleus. Nucleocytoplasmic shuttling protein that can transiently enter the cytoplasm in mammalian cells in a XPO1/CRM1-dependent manner |
| Entrez Gene | 28960 |
|---|---|
| Nucleotide Accession | NP_054745.1 |
Application
DCPS Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the DCPS antibody detected the DCPS protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
