Application
Western blot analysis of DCTN1 Antibody (N-term) in Hela cell line lysates (35ug/lane). DCTN1 (arrow) was detected using the purified Pab.Application
DCTN1 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DCTN1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
DCTN1 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | DCTN1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DCTN1; Dynactin subunit 1; 150 kDa dynein-associated polypeptide; DAP-150; p135; p150-glued |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | DCTN1 Dynactin subunit 1 150 kDa dynein-associated polypeptide DAP-150 p135 p150-glued |
|---|---|
| Molecular Weight(MW) | 141695 Da |
| Function | Required for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein- dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles |
| Tissue Specificity | Brain. |
| Cellular Localization | Cytoplasm. Cytoplasm, cytoskeleton. Note=Colocalizes with microtubules |
| Entrez Gene | 1639 |
|---|
Application
Western blot analysis of DCTN1 Antibody (N-term) in Hela cell line lysates (35ug/lane). DCTN1 (arrow) was detected using the purified Pab.
Application
DCTN1 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DCTN1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
DCTN1 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
