Application
Formalin-fixed and paraffin-embedded mouse kidney reacted with DDAH1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of DDAH1 Antibody (C-term) in mouse kidney tissue lysates (35ug/lane). DDAH1 (arrow) was detected using the purified Pab.Application
DDAH1 Antibody (C-term) flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of DDAH1 Antibody (C-term)(Cat#AP2898b) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | DDAH1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DDAH1; DDAH; N(G), N(G)-dimethylarginine dimethylaminohydrolase 1; DDAHI; Dimethylargininase-1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCWBFCIF |
| IHC:1:50~100 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | DDAH |
|---|---|
| Alternative Names | DDAH1 DDAH N(G) N(G)-dimethylarginine dimethylaminohydrolase 1 DDAHI Dimethylargininase-1 |
| Molecular Weight(MW) | 31122 Da |
| Function | Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS Has therefore a role in the regulation of nitric oxide generation |
| Tissue Specificity | Detected in brain, liver, kidney and pancreas, and at low levels in skeletal muscle |
| Entrez Gene | 23576 |
|---|
Application
Formalin-fixed and paraffin-embedded mouse kidney reacted with DDAH1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of DDAH1 Antibody (C-term) in mouse kidney tissue lysates (35ug/lane). DDAH1 (arrow) was detected using the purified Pab.
Application
DDAH1 Antibody (C-term) flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of DDAH1 Antibody (C-term)(Cat#AP2898b) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | IHC:1:50~100 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
