Application
DDAH2 Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the DDAH2 antibody detected the DDAH2 protein (arrow).Application
DDAH2 antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DDAH2 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
DDAH2 Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | DDAH2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DDAH2; DDAH; G6A; NG30; N(G), N(G)-dimethylarginine dimethylaminohydrolase 2; DDAHII; Dimethylargininase-2; Protein G6a; S-phase protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | DDAH G6A NG30 |
|---|---|
| Alternative Names | DDAH2 DDAH G6A NG30 N(G) N(G)-dimethylarginine dimethylaminohydrolase 2 DDAHII Dimethylargininase-2 Protein G6a S-phase protein |
| Molecular Weight(MW) | 29644 Da |
| Function | Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS Has therefore a role in the regulation of nitric oxide generation |
| Tissue Specificity | Detected in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas, and at very low levels in brain |
| Cellular Localization | Cytoplasm. Mitochondrion. Note=Translocates from cytosol to mitochondrion upon IL-1beta stimulation in chondrocytes |
| Entrez Gene | 23564 |
|---|---|
| Nucleotide Accession | NP_039268.1 |
Application
DDAH2 Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the DDAH2 antibody detected the DDAH2 protein (arrow).
Application
DDAH2 antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DDAH2 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
DDAH2 Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
