Application
Western blot analysis of DDB1 Antibody (Y660) in HL-60 cell line lysates (35ug/lane). DDB1 (arrow) was detected using the purified Pab.Application
DDB1 Antibody (Y660) IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DDB1 Antibody (Y660) for immunohistochemistry. Clinical relevance has not been evaluated.Application
DDB1 Antibody (Y660) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | DDB1 Antibody (Y660) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DDB1; XAP1; DNA damage-binding protein 1; DDB p127 subunit; DNA damage-binding protein a; Damage-specific DNA-binding protein 1; HBV X-associated protein 1; UV-damaged DNA-binding factor; UV-damaged DNA-binding protein 1; XPE-binding factor; Xeroderma pigm |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | XAP1 |
|---|---|
| Alternative Names | DDB1 XAP1 DNA damage-binding protein 1 DDB p127 subunit DNA damage-binding protein a Damage-specific DNA-binding protein 1 HBV X-associated protein 1 UV-damaged DNA-binding factor UV-damaged DNA-binding protein 1 XPE-binding factor Xeroderma pigm |
| Molecular Weight(MW) | 126968 Da |
| Function | Required for DNA repair. Binds to DDB2 to form the UV- damaged DNA-binding protein complex (the UV-DDB complex). The UV- DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin- protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR). May also play a role in ubiquitination of CDKN1B/p27kip when associated with CUL4 and SKP2 |
| Tissue Specificity | This DDB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 646~676 amino acids from the Y660 region of human DDB1. |
| Cellular Localization | Cytoplasm. Nucleus. Note=Primarily cytoplasmic. Translocates to the nucleus following UV irradiation and subsequently accumulates at sites of DNA damage |
| Entrez Gene | 1642 |
|---|
Application
Western blot analysis of DDB1 Antibody (Y660) in HL-60 cell line lysates (35ug/lane). DDB1 (arrow) was detected using the purified Pab.
Application
DDB1 Antibody (Y660) IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DDB1 Antibody (Y660) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
DDB1 Antibody (Y660) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
