Application
Western blot analysis of DDHD2 Antibody (N-term) in K562 cell line lysates (35ug/lane).DDHD2 (arrow) was detected using the purified Pab.Application
DDHD2 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DDHD2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
DDHD2 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | DDHD2 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DDHD2; KIAA0725; SAMWD1; Phospholipase DDHD2; DDHD domain-containing protein 2; SAM, WWE and DDHD domain-containing protein 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0725 SAMWD1 |
|---|---|
| Alternative Names | DDHD2 KIAA0725 SAMWD1 Phospholipase DDHD2 DDHD domain-containing protein 2 SAM WWE and DDHD domain-containing protein 1 |
| Molecular Weight(MW) | 81032 Da |
| Function | Phospholipase that hydrolyzes preferentially phosphatidic acid, including 1,2-dioleoyl-sn-phosphatidic acid, and phosphatidylethanolamine. Specifically binds to phosphatidylinositol 3-phosphate (PI(3)P), phosphatidylinositol 4- phosphate (PI(4)P), phosphatidylinositol 5-phosphate (PI(5)P) and possibly phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2). May be involved in the maintenance of the endoplasmic reticulum and/or Golgi structures. May regulate the transport between Golgi apparatus and plasma membrane |
| Tissue Specificity | Widely expressed (at protein level). |
| Cellular Localization | Cytoplasm, cytosol. Endoplasmic reticulum- Golgi intermediate compartment. Golgi apparatus, cis-Golgi network. Note=Cycles between the Golgi apparatus and the cytosol DDHD2 recruitment to the Golgi/endoplasmic reticulum-Golgi intermediate compartment (ERGIC) is regulated by the levels of phosphoinositides, including PI(4)P |
| Entrez Gene | 23259 |
|---|
Application
Western blot analysis of DDHD2 Antibody (N-term) in K562 cell line lysates (35ug/lane).DDHD2 (arrow) was detected using the purified Pab.
Application
DDHD2 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DDHD2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
DDHD2 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
