Application
DDX11 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the DDX11 antibody detected the DDX11 protein (arrow).Application
DDX11 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | DDX11 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DDX11; CHL1; CHLR1; KRG2; Probable ATP-dependent RNA helicase DDX11; CHL1-related protein 1; DEAD/H box protein 11; Keratinocyte growth factor-regulated gene 2 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CHL1 CHLR1 KRG2 |
|---|---|
| Alternative Names | DDX11 CHL1 CHLR1 KRG2 Probable ATP-dependent RNA helicase DDX11 CHL1-related protein 1 DEAD/H box protein 11 Keratinocyte growth factor-regulated gene 2 protein |
| Molecular Weight(MW) | 108313 Da |
| Function | DNA helicase involved in cellular proliferation. Possesses DNA-dependent ATPase and helicase activities. This helicase translocates on single-stranded DNA in the 5' to 3' direction in the presence of ATP and, to a lesser extent, dATP Its unwinding activity requires a 5'-single-stranded region for helicase loading, since flush-ended duplex structures do not support unwinding. The helicase activity is capable of displacing duplex regions up to 100 bp, which can be extended to 500 bp by RPA or the cohesion establishment factor, the Ctf18-RFC (replication factor C) complex activities. Stimulates the flap endonuclease activity of FEN1. Required for normal sister chromatid cohesion. Required for recruitment of bovine papillomavirus type 1 regulatory protein E2 to mitotic chrmosomes and for viral genome maintenance. Required for maintaining the chromosome segregation and is essential for embryonic development and the prevention of aneuploidy. May function during either S, G2, or M phase of the cell cycle. Binds to both single- and double-stranded DNA |
| Tissue Specificity | Highly expressed in spleen, B-cells, thymus, testis, ovary, small intestine, and pancreas. Very low expression seen in the brain. Expressed in dividing cells and/or cells undergoing high levels of recombination. No expression is seen in cells signaled to terminally differentiate. Expressed in keratinocyte growth factor-stimulated cells but not in serum, EGF and IL1-beta-treated keratinocytes |
| Cellular Localization | Nucleus. Nucleus, nucleolus. Note=During the early stages of mitosis, localizes to condensed chromatin and is released from the chromatin with progression to metaphase. Also localizes to the spindle poles throughout mitosis and at the midbody at later stages of mitosis (metaphase to telophase). Co- localizes with bovine papillomavirus type 1 regulatory protein E2 at early stages of mitosis |
| Entrez Gene | 1663 |
|---|---|
| Nucleotide Accession | NP_689651.1 NP_004390.3 |
Application
DDX11 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the DDX11 antibody detected the DDX11 protein (arrow).
Application
DDX11 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
