WB
Western blot analysis of DDX11 expression in HeLa (A) and F9 (B) whole cell lysate.ICC/IF
Immunofluorescence staining of Hep G2 cells fixed in formalin reveals nuclear localization.| Product Name | Mouse Monoclonal antibody to DDX11 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | raised against amino acids 405-704 mapping within an internal region of DDX11 of human origin. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG1, k |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| WB:1:100-1:1000 ICC/IF:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DDX11 |
|---|---|
| Gene Synonyms | CHL1 KRG2 WABS CHLR1 |
| Gene Full Name | DEAD/H-box helicase 11 |
| Gene Summary | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an enzyme that possesses both ATPase and DNA helicase activities. This gene is a homolog of the yeast CHL1 gene, and may function to maintain chromosome transmission fidelity and genome stability. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 112 kDa |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of DDX11 expression in HeLa (A) and F9 (B) whole cell lysate.
ICC/IF
Immunofluorescence staining of Hep G2 cells fixed in formalin reveals nuclear localization.| Application Notes | WB:1:100-1:1000 ICC/IF:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. |
| Storage Buffer | Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
