Application
Western blot analysis of Deaf1(arrow) using rabbit polyclonal Deaf1 Antibody (Human C-term) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Deaf1 gene (Lane 2) (Origene Technologies).| Product Name | Deaf1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Deaf1; Deformed epidermal autoregulatory factor 1 homolog; Nuclear DEAF-1-related transcriptional regulator |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:50~100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Deaf1 Deformed epidermal autoregulatory factor 1 homolog Nuclear DEAF-1-related transcriptional regulator |
|---|---|
| Molecular Weight(MW) | 59633 Da |
| Function | Transcription factor that binds to sequence with multiple copies of 5'-TTC[CG]G-3' present in its own promoter and that of the HNRPA2B1 gene. Down-regulates transcription of these genes. Binds to the retinoic acid response element (RARE) 5'- AGGGTTCACCGAAAGTTCA-3'. Activates the proenkephalin gene independently of promoter binding, probably through protein- protein interaction (By similarity). Regulates epithelial cell proliferation and side-branching in the mammary gland. Required for neural tube closure and skeletal patterning. Controls the expression of peripheral tissue antigens in pancreatic lymph nodes. Isoform 1 displays greater transcriptional activity than isoform 2. Isoform 2 may inhibit transcriptional activity of isoform 1 by interacting with it and retaining it in the cytoplasm |
| Tissue Specificity | Ubiquitously expressed during embryogenesis, with higher expression in regions of the central nervous system, dorsal root ganglia, submandibular gland, epidermis and breast. In 12-week-old NOD mice, expression of isoform 2 is sevenfold higher in lymph node stromal elements than in T-cells and tenfold higher than in B-cells |
| Cellular Localization | Isoform 1: Nucleus. Cytoplasm (By similarity) |
| Entrez Gene | 54006 |
|---|
Application
Western blot analysis of Deaf1(arrow) using rabbit polyclonal Deaf1 Antibody (Human C-term) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Deaf1 gene (Lane 2) (Origene Technologies).| Application Notes | WB:1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
