Application
DEM1 Antibody (Center) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the DEM1 antibody detected the DEM1 protein (arrow).Application
DEM1 Antibody (Center) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the DEM1 antibody detected the DEM1 protein (arrow).Application
DEM1 Antibody (Center) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the DEM1 antibody detected the DEM1 protein (arrow).Application
DEM1 Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | DEM1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DEM1; C1orf176; Probable exonuclease V; Defects in morphology protein 1 homolog |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | C1orf176 DEM1 |
|---|---|
| Alternative Names | DEM1 C1orf176 Probable exonuclease V Defects in morphology protein 1 homolog |
| Molecular Weight(MW) | 41816 Da |
| Function | Single-stranded DNA (ssDNA) bidirectional exonuclease involved in DNA repair. Probably involved in DNA repair following ultraviolet (UV) irradiation and interstrand cross-links (ICLs) damage. Has both 5'-3' and 3'-5' exonuclease activities with a strong preference for 5'-ends. Acts as a sliding exonuclease that loads at ssDNA ends and then slides along the ssDNA prior to cutting; however the sliding and the 3'-5' exonuclease activities are abolished upon binding to the replication protein A (RPA) complex that enforces 5'-directionality activity |
| Tissue Specificity | This DEM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 173-201 amino acids from the Central region of human DEM1. |
| Cellular Localization | Nucleus. Cytoplasm, cytosol. Note=Localizes to repair foci in response to DNA damage |
| Entrez Gene | 64789 |
|---|---|
| Nucleotide Accession | NP_073611.1 |
Application
DEM1 Antibody (Center) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the DEM1 antibody detected the DEM1 protein (arrow).
Application
DEM1 Antibody (Center) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the DEM1 antibody detected the DEM1 protein (arrow).
Application
DEM1 Antibody (Center) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the DEM1 antibody detected the DEM1 protein (arrow).
Application
DEM1 Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
