Application
Western blot analysis of DEN1A Antibody (C-term) in K562 cell line lysates (35ug/lane). DEN1A (arrow) was detected using the purified Pab.Application
DEN1A Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DEN1A Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
DEN1A Antibody (C-term) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | DEN1A Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DENND1A; FAM31A; KIAA1608; DENN domain-containing protein 1A; Connecdenn 1; Protein FAM31A |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | FAM31A KIAA1608 |
|---|---|
| Alternative Names | DENND1A FAM31A KIAA1608 DENN domain-containing protein 1A Connecdenn 1 Protein FAM31A |
| Molecular Weight(MW) | 110577 Da |
| Function | Guanine nucleotide exchange factor (GEF) regulating clathrin-mediated endocytosis through RAB35 activation. Promotes the exchange of GDP to GTP, converting inactive GDP-bound RAB35 into its active GTP-bound form. Regulates clathrin-mediated endocytosis of synaptic vesicles |
| Tissue Specificity | This DEN1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 583~613 amino acids from the C-terminal region of human DEN1A. |
| Cellular Localization | Cytoplasmic vesicle, clathrin-coated vesicle membrane; Peripheral membrane protein. Cell junction, synapse, presynaptic cell membrane |
| Entrez Gene | 57706 |
|---|
Application
Western blot analysis of DEN1A Antibody (C-term) in K562 cell line lysates (35ug/lane). DEN1A (arrow) was detected using the purified Pab.
Application
DEN1A Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DEN1A Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
DEN1A Antibody (C-term) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
