Application
DEN2D Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the DEN2D antibody detected the DEN2D protein (arrow).Application
DEN2D antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of the DEN2D antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
DEN2D Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | DEN2D Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DENND2D; DENN domain-containing protein 2D |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | DENND2D DENN domain-containing protein 2D |
|---|---|
| Molecular Weight(MW) | 53672 Da |
| Function | Guanine nucleotide exchange factor (GEF) which may activate RAB9A and RAB9B. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP- bound form |
| Tissue Specificity | This DEN2D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 450-479 amino acids from the C-terminal region of human DEN2D. |
| Entrez Gene | 79961 |
|---|---|
| Nucleotide Accession | NP_079177.2 |
Application
DEN2D Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the DEN2D antibody detected the DEN2D protein (arrow).
Application
DEN2D antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of the DEN2D antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
DEN2D Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
