Application
DENND1B Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the DENND1B antibody detected the DENND1B protein (arrow).Application
DENND1B antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DENND1B antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
DENND1B Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | DENND1B Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DENND1B; C1orf218; FAM31B; DENN domain-containing protein 1B; Connecdenn 2; Protein FAM31B |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | C1orf218 FAM31B |
|---|---|
| Alternative Names | DENND1B C1orf218 FAM31B DENN domain-containing protein 1B Connecdenn 2 Protein FAM31B |
| Molecular Weight(MW) | 48260 Da |
| Function | Guanine nucleotide exchange factor (GEF) for RAB35. Promotes the exchange of GDP to GTP, converting inactive GDP-bound RAB35 into its active GTP-bound form. May play a role in clathrin- mediated endocytosis |
| Tissue Specificity | Highly expressed in dendritic and natural killer cells and at lower levels in other myeloid lineage cells and in pituitary. Significantly up-regulated in effector memory T- cells as compared with naive T-cells |
| Cellular Localization | Cytoplasm, cytosol (By similarity). Cytoplasmic vesicle, clathrin-coated vesicle (By similarity) |
| Entrez Gene | 163486 |
|---|---|
| Nucleotide Accession | NP_001182144.1 NP_001182145.1 |
Application
DENND1B Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the DENND1B antibody detected the DENND1B protein (arrow).
Application
DENND1B antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DENND1B antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
DENND1B Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
