Application
Western blot analysis of anti-DET1 ANtibody (C-term) in CEM cell line lysates (35ug/lane).Det1(arrow) was detected using the purified Pab (1:60dilution).Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with Det1 (Human C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | DET1 ANtibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DET1; DET1 homolog; De-etiolated-1 homolog |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | DET1 DET1 homolog De-etiolated-1 homolog |
|---|---|
| Molecular Weight(MW) | 63848 Da |
| Function | Component of the E3 ubiquitin ligase DCX DET1-COP1 complex, which is required for ubiquitination and subsequent degradation of target proteins. The complex is involved in JUN ubiquitination and degradation |
| Tissue Specificity | Highly expressed in the ovary, some lymphoid organs and resting leukocytes |
| Cellular Localization | Nucleus (Probable). |
Application
Western blot analysis of anti-DET1 ANtibody (C-term) in CEM cell line lysates (35ug/lane).Det1(arrow) was detected using the purified Pab (1:60dilution).
Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with Det1 (Human C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
