Application
DGKQ Antibody (R927) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the DGKQ antibody detected the DGKQ protein (arrow).Application
DGKQ Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DGKQ Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
DGKQ Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | DGKQ Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DGKQ; DAGK4; Diacylglycerol kinase theta; Diglyceride kinase theta |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | DAGK4 |
|---|---|
| Alternative Names | DGKQ DAGK4 Diacylglycerol kinase theta Diglyceride kinase theta |
| Molecular Weight(MW) | 101155 Da |
| Function | Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). May regulate the activity of protein kinase C by controlling the balance between these two signaling lipids. Activated in the nucleus in response to alpha-thrombin and nerve growth factor (By similarity). May be involved in cAMP- induced activation of NR5A1 and subsequent steroidogenic gene transcription by delivering PA as ligand for NR5A1. Acts synergistically with NR5A1 on CYP17 transcriptional activity |
| Tissue Specificity | This DGKQ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 913~942 amino acids from the C-terminal region of human DGKQ. |
| Cellular Localization | Cytoplasm. Cell membrane. Cytoplasm, cytoskeleton. Nucleus. Nucleus speckle. Note=Translocates to the nucleus in response to thrombin stimulation (By similarity) Translocates to the plasma membrane in response to steroid hormone receptor stimulation. Translocation to the plasma membrane is dependent on G-protein coupled receptor stimulation and subsequent activation of PRKCE and probably PRKCH |
| Entrez Gene | 1609 |
|---|
Application
DGKQ Antibody (R927) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the DGKQ antibody detected the DGKQ protein (arrow).
Application
DGKQ Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DGKQ Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
DGKQ Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
