Application
Western blot analysis of DHFR Antibody (Center) in Y79 cell line lysates (35ug/lane). DHFR (arrow) was detected using the purified Pab.Application
DHFR Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DHFR Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Western blot analysis of DHFR (arrow) using rabbit polyclonal DHFR Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DHFR gene.Application
Confocal immunofluorescent analysis of DHFR Antibody (Center)(Cat#AP7378c) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
DHFR Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Product Name | DHFR Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DHFR; Dihydrofolate reductase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | DHFR Dihydrofolate reductase |
|---|---|
| Molecular Weight(MW) | 21453 Da |
| Function | Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1 |
| Tissue Specificity | Widely expressed in fetal and adult tissues, including throughout the fetal and adult brains and whole blood Expression is higher in the adult brain than in the fetal brain |
| Entrez Gene | 1719 |
|---|
Application
Western blot analysis of DHFR Antibody (Center) in Y79 cell line lysates (35ug/lane). DHFR (arrow) was detected using the purified Pab.
Application
DHFR Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DHFR Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Western blot analysis of DHFR (arrow) using rabbit polyclonal DHFR Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DHFR gene.
Application
Confocal immunofluorescent analysis of DHFR Antibody (Center)(Cat#AP7378c) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
DHFR Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
