Application
DHX58 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the DHX58 antibody detected the DHX58 protein (arrow).Application
DHX58 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DHX58 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | DHX58 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DHX58; D11LGP2E; LGP2; Probable ATP-dependent RNA helicase DHX58; Probable ATP-dependent helicase LGP2; Protein D11Lgp2 homolog; RIG-I-like receptor 3; RIG-I-like receptor LGP2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | D11LGP2E LGP2 |
|---|---|
| Alternative Names | DHX58 D11LGP2E LGP2 Probable ATP-dependent RNA helicase DHX58 Probable ATP-dependent helicase LGP2 Protein D11Lgp2 homolog RIG-I-like receptor 3 RIG-I-like receptor LGP2 |
| Molecular Weight(MW) | 76613 Da |
| Function | Acts as a regulator of DDX58/RIG-I and IFIH1/MDA5 mediated antiviral signaling. Cannot initiate antiviral signaling as it lacks the CARD domain required for activating MAVS/IPS1- dependent signaling events. Can have both negative and positive regulatory functions related to DDX58/RIG-I and IFIH1/MDA5 signaling and this role in regulating signaling may be complex and could probably depend on characteristics of the infecting virus or target cells, or both. Its inhibitory action on DDX58/RIG-I signaling may involve the following mechanisms: competition with DDX58/RIG-I for binding to the viral RNA, binding to DDX58/RIG-I and inhibiting its dimerization and interaction with MAVS/IPS1, competing with IKBKE in its binding to MAVS/IPS1 thereby inhibiting activation of interferon regulatory factor 3 (IRF3) Its positive regulatory role may involve unwinding or stripping nucleoproteins of viral RNA thereby facilitating their recognition by DDX58/RIG-I and IFIH1/MDA5. Involved in the innate immune response to various RNA viruses and some DNA viruses such as poxviruses, and also to the bacterial pathogen Listeria monocytogenes. Can bind both ssRNA and dsRNA, with a higher affinity for dsRNA. Shows a preference to 5'-triphosphorylated RNA, although it can recognize RNA lacking a 5'-triphosphate |
| Tissue Specificity | This DHX58 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 48-77 amino acids from the N-terminal region of human DHX58. |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 79132 |
|---|---|
| Nucleotide Accession | NP_077024.2 |
Application
DHX58 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the DHX58 antibody detected the DHX58 protein (arrow).
Application
DHX58 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DHX58 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
