Application
Western blot analysis of DIO2 Antibody (Center) in MCF-7 cell line lysates (35ug/lane). DIO2 (arrow) was detected using the purified Pab.Application
DIO2 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded mouse brain followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the mouse DIO2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
DIO2 Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | DIO2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DIO2; ITDI2; TXDI2; Type II iodothyronine deiodinase; 5DII; DIOII; Type 2 DI; Type-II 5'-deiodinase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ITDI2 TXDI2 |
|---|---|
| Alternative Names | DIO2 ITDI2 TXDI2 Type II iodothyronine deiodinase 5DII DIOII Type 2 DI Type-II 5'-deiodinase |
| Molecular Weight(MW) | 30552 Da |
| Function | Responsible for the deiodination of T4 (3,5,3',5'- tetraiodothyronine) into T3 (3,5,3'-triiodothyronine). Essential for providing the brain with appropriate levels of T3 during the critical period of development |
| Tissue Specificity | Heart, skeletal muscle, placenta, fetal brain and several regions of the adult brain |
| Cellular Localization | Membrane; Single-pass membrane protein (Potential) |
| Entrez Gene | 1734 |
|---|
Application
Western blot analysis of DIO2 Antibody (Center) in MCF-7 cell line lysates (35ug/lane). DIO2 (arrow) was detected using the purified Pab.
Application
DIO2 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded mouse brain followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the mouse DIO2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
DIO2 Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
