Application
DLAT Antibody (C-term) western blot analysis in K562(lane 1),HepG2(lane 2),Jurkat(lane 3) cell line lysates (35ug/lane).This demonstrates the DLAT antibody detected the DLAT protein (arrow).Application
DLAT Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DLAT Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
DLAT Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | DLAT Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DLAT; DLTA; Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial; 70 kDa mitochondrial autoantigen of primary biliary cirrhosis; Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase com |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | DLTA |
|---|---|
| Alternative Names | DLAT DLTA Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex mitochondrial 70 kDa mitochondrial autoantigen of primary biliary cirrhosis Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase com |
| Molecular Weight(MW) | 68997 Da |
| Function | The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle |
| Tissue Specificity | This DLAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 587-617 amino acids from the C-terminal region of human DLAT. |
| Cellular Localization | Mitochondrion matrix. |
| Entrez Gene | 1737 |
|---|---|
| Nucleotide Accession | NP_001922.2 |
Application
DLAT Antibody (C-term) western blot analysis in K562(lane 1),HepG2(lane 2),Jurkat(lane 3) cell line lysates (35ug/lane).This demonstrates the DLAT antibody detected the DLAT protein (arrow).
Application
DLAT Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DLAT Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
DLAT Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
