Application
Western blot analysis of DLX5 (arrow) using rabbit polyclonal DLX5 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DLX5 gene.Application
DLX5 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the DLX5 antibody detected the DLX5 protein (arrow).Application
DLX5 Antibody (Center) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the DLX5 antibody detected the DLX5 protein (arrow).Application
DLX5 Antibody (Center) (AP14365c)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DLX5 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | DLX5 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DLX5; Homeobox protein DLX-5 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | DLX5 Homeobox protein DLX-5 |
|---|---|
| Molecular Weight(MW) | 31540 Da |
| Function | Transcriptional factor involved in bone development. Acts as an immediate early BMP-responsive transcriptional activator essential for osteoblast differentiation. Stimulates ALPL promoter activity in a RUNX2-independent manner during osteoblast differentiation. Stimulates SP7 promoter activity during osteoblast differentiation. Promotes cell proliferation by up-regulating MYC promoter activity. Involved as a positive regulator of both chondrogenesis and chondrocyte hypertrophy in the endochondral skeleton. Binds to the homeodomain-response element of the ALPL and SP7 promoter. Binds to the MYC promoter Requires the 5'-TAATTA-3' consensus sequence for DNA-binding |
| Tissue Specificity | This DLX5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 99-128 amino acids from the Central region of human DLX5. |
| Cellular Localization | Nucleus (By similarity). |
| Entrez Gene | 1749 |
|---|---|
| Nucleotide Accession | NP_005212.1 |
Application
Western blot analysis of DLX5 (arrow) using rabbit polyclonal DLX5 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DLX5 gene.
Application
DLX5 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the DLX5 antibody detected the DLX5 protein (arrow).
Application
DLX5 Antibody (Center) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the DLX5 antibody detected the DLX5 protein (arrow).
Application
DLX5 Antibody (Center) (AP14365c)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DLX5 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
