Application
Western blot analysis of DLST Antibody (Center) in HepG2 cell line lysates (35ug/lane). DLST (arrow) was detected using the purified Pab.(2ug/ml)Application
Western blot analysis of DLST Antibody (Center) in mouse heart tissue lysates (35ug/lane). DLST (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with DLST Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of DLST Antibody (Center)(Cat#AP2899c) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
DLST Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | DLST Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DLST; DLTS; Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial; 2-oxoglutarate dehydrogenase complex component E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | DLTS |
|---|---|
| Alternative Names | DLST DLTS Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex mitochondrial 2-oxoglutarate dehydrogenase complex component E2 Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase |
| Molecular Weight(MW) | 48755 Da |
| Function | The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3) |
| Tissue Specificity | This DLST antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 209~239 amino acids from the Center region of human DLST. |
| Cellular Localization | Mitochondrion. |
| Entrez Gene | 1743 |
|---|
Application
Western blot analysis of DLST Antibody (Center) in HepG2 cell line lysates (35ug/lane). DLST (arrow) was detected using the purified Pab.(2ug/ml)
Application
Western blot analysis of DLST Antibody (Center) in mouse heart tissue lysates (35ug/lane). DLST (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with DLST Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of DLST Antibody (Center)(Cat#AP2899c) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
DLST Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
