Application
DMRTA2 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the DMRTA2 antibody detected the DMRTA2 protein (arrow).Application
Fluorescent confocal image of U251 cell stained with DMRTA2 Antibody (C-term)(Cat#AP19969b).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with DMRTA2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). DMRTA2 immunoreactivity is localized to Microtubules significantly.| Product Name | DMRTA2 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DMRTA2; DMRT5; Doublesex- and mab-3-related transcription factor A2; Doublesex- and mab-3-related transcription factor 5 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | DMRT5 |
|---|---|
| Alternative Names | DMRTA2 DMRT5 Doublesex- and mab-3-related transcription factor A2 Doublesex- and mab-3-related transcription factor 5 |
| Molecular Weight(MW) | 53356 Da |
| Function | May be involved in sexual development. |
| Tissue Specificity | Expressed in testis. |
| Cellular Localization | Nucleus (Potential). |
| Entrez Gene | 63950 |
|---|---|
| Nucleotide Accession | NP_115486.1 |
Application
DMRTA2 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the DMRTA2 antibody detected the DMRTA2 protein (arrow).
Application
Fluorescent confocal image of U251 cell stained with DMRTA2 Antibody (C-term)(Cat#AP19969b).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with DMRTA2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). DMRTA2 immunoreactivity is localized to Microtubules significantly.| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
