Application
DNA2 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the DNA2 antibody detected the DNA2 protein (arrow).Application
DNA2 antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human thymus carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DNA2 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
DNA2 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | DNA2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DNA2; DNA2L; KIAA0083; DNA2-like helicase; DNA replication ATP-dependent helicase-like homolog |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | DNA2L KIAA0083 |
|---|---|
| Alternative Names | DNA2 DNA2L KIAA0083 DNA2-like helicase DNA replication ATP-dependent helicase-like homolog |
| Molecular Weight(MW) | 120415 Da |
| Function | Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double- strand break (DSB) repair: recruited by BLM and mediates the cleavage of 5'-ssDNA, while the 3'-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing. Possesses different enzymatic activities, such as single-stranded DNA (ssDNA)- dependent ATPase, 5'-3' helicase and endonuclease activities While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is subject to debate. According to various reports, the helicase activity is weak and its function remains largely unclear. Helicase activity may promote the motion of DNA2 on the flap, helping the nuclease function |
| Tissue Specificity | This DNA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 337-366 amino acids from the Central region of human DNA2. |
| Cellular Localization | Nucleus. Mitochondrion. Note=Was initially reported to be exclusively mitochondrial (PubMed:18995831) However, it was later shown to localize both in mitochondrion and nucleus (PubMed:19487465) |
| Entrez Gene | 1763 |
|---|---|
| Nucleotide Accession | NP_001073918.1 |
Application
DNA2 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the DNA2 antibody detected the DNA2 protein (arrow).
Application
DNA2 antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human thymus carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DNA2 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
DNA2 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
