Application
Western blot analysis of DNAJB6 Antibody (Center) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in Hela cell line lysate. DNAJB6 Antibody (Center) (arrow) was detected using the purified Pab (1:60/250 dilution).Application
DNAJB6 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DNAJB6 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
DNAJB6 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | DNAJB6 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DNAJB6; HSJ2; MRJ; MSJ1; DnaJ homolog subfamily B member 6; HHDJ1; Heat shock protein J2; MSJ-1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HSJ2 MRJ MSJ1 |
|---|---|
| Alternative Names | DNAJB6 HSJ2 MRJ MSJ1 DnaJ homolog subfamily B member 6 HHDJ1 Heat shock protein J2 MSJ-1 |
| Molecular Weight(MW) | 36087 Da |
| Function | Plays an indispensable role in the organization of KRT8/KRT18 filaments. Acts as an endogenous molecular chaperone for neuronal proteins including huntingtin. Suppresses aggregation and toxicity of polyglutamine-containing, aggregation-prone proteins. Isoform B but not isoform A inhibits huntingtin aggregation. Has a stimulatory effect on the ATPase activity of HSP70 in a dose-dependent and time-dependent manner and hence acts as a co-chaperone of HSP70. Also reduces cellular toxicity and caspase-3 activity |
| Tissue Specificity | Widely expressed. Highest levels in testis and brain, and lower levels in heart, spleen, intestine, ovary, placenta, lung, kidney, pancreas, thymus, prostate, skeletal muscle, liver and leukocytes. In testis, expressed in germ cells in the earlier stages of differentiation pathway as well as in spermatids. In brain, expressed at a higher level in hippocampus and thalamus and a lower level in amygdala, substantia nigra, corpus callosum and caudate nucleus |
| Cellular Localization | Cytoplasm, perinuclear region. Nucleus. Cytoplasm, myofibril, sarcomere, Z line |
| Entrez Gene | 10049 |
|---|---|
| Nucleotide Accession | NP_490647.1 NP_005485.1 |
Application
Western blot analysis of DNAJB6 Antibody (Center) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in Hela cell line lysate. DNAJB6 Antibody (Center) (arrow) was detected using the purified Pab (1:60/250 dilution).
Application
DNAJB6 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DNAJB6 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
DNAJB6 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
