Application
Western blot analysis of DNASE2 Antibody (Center) in Y79 cell line lysates (35ug/lane). DNASE2 (arrow) was detected using the purified Pab.Application
DNASE2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DNASE2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
DNASE2 Antibody (Center) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of DNASE2 Antibody (Center)(Cat#AP8894c) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | DNASE2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DNASE2; DNASE2A; DNL2; Deoxyribonuclease-2-alpha; Acid DNase; Deoxyribonuclease II alpha; Lysosomal DNase II; R31240_2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | DNASE2A DNL2 |
|---|---|
| Alternative Names | DNASE2 DNASE2A DNL2 Deoxyribonuclease-2-alpha Acid DNase Deoxyribonuclease II alpha Lysosomal DNase II R31240_2 |
| Molecular Weight(MW) | 39581 Da |
| Function | Hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. Plays a major role in the degradation of nuclear DNA in cellular apoptosis during development. Necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells |
| Tissue Specificity | This DNASE2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30~59 amino acids from the Center region of human DNASE2. |
| Cellular Localization | Lysosome. |
| Entrez Gene | 1777 |
|---|
Application
Western blot analysis of DNASE2 Antibody (Center) in Y79 cell line lysates (35ug/lane). DNASE2 (arrow) was detected using the purified Pab.
Application
DNASE2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DNASE2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
DNASE2 Antibody (Center) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of DNASE2 Antibody (Center)(Cat#AP8894c) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
