Application
Western blot analysis of anti-DNMT3A Antibody (Center D472) in Jurkat cell line lysates (35ug/lane).DNMT3A(arrow) was detected using the purified Pab (1:60dilution).Application
Formalin-fixed and paraffin-embedded human kidney tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | Dnmt3a Antibody (Center D472) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DNMT3A; DNA (cytosine-5)-methyltransferase 3A; DNA methyltransferase HsaIIIA |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | DNMT3A DNA (cytosine-5)-methyltransferase 3A DNA methyltransferase HsaIIIA |
|---|---|
| Molecular Weight(MW) | 101858 Da |
| Function | Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity (By similarity) |
| Tissue Specificity | Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung |
| Cellular Localization | Nucleus. Cytoplasm. Note=Accumulates in the major satellite repeats at pericentric heterochromatin (By similarity) |
| Entrez Gene | 1788 |
|---|
Application
Western blot analysis of anti-DNMT3A Antibody (Center D472) in Jurkat cell line lysates (35ug/lane).DNMT3A(arrow) was detected using the purified Pab (1:60dilution).
Application
Formalin-fixed and paraffin-embedded human kidney tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
