Application
A. Western blot analysis on (A) A549, (B) HeLa and (C) A431 cell lysates using anti-Dnmt3b RabMAb (Cat. #AJ1244a), dilution: 1:5000.| Product Name | Dnmt3b Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DNMT3B, DNA (cytosine-5)-methyltransferase 3B, DNA methyltransferase HsaIIIB |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR3523 |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000~10000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | DNMT3B DNA (cytosine-5)-methyltransferase 3B DNA methyltransferase HsaIIIB |
|---|---|
| Molecular Weight(MW) | 95751 Da |
| Function | Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co- repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing (By similarity). In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9 Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs. Function as transcriptional corepressor by associating with ZHX1 |
| Tissue Specificity | Ubiquitous; highly expressed in fetal liver, heart, kidney, placenta, and at lower levels in spleen, colon, brain, liver, small intestine, lung, peripheral blood mononuclear cells, and skeletal muscle. Isoform 1 is expressed in all tissues except brain, skeletal muscle and PBMC, 3 is ubiquitous, 4 is expressed in all tissues except brain, skeletal muscle, lung and prostate and 5 is detectable only in testis and at very low level in brain and prostate |
| Cellular Localization | Nucleus. |
| Entrez Gene | 1789 |
|---|
Application
A. Western blot analysis on (A) A549, (B) HeLa and (C) A431 cell lysates using anti-Dnmt3b RabMAb (Cat. #AJ1244a), dilution: 1:5000.| Application Notes | WB:1:1000~10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
