Application
DNMT3A Antibody (Center R478) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the DNMT3A antibody detected the DNMT3A protein (arrow).Application
DNMT3A Antibody (Center R478WMutant) (AP14633c)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DNMT3A Antibody (Center R478WMutant) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | DNMT3A Antibody (Center R478) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DNMT3A; DNA (cytosine-5)-methyltransferase 3A; DNA methyltransferase HsaIIIA |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | DNMT3A DNA (cytosine-5)-methyltransferase 3A DNA methyltransferase HsaIIIA |
|---|---|
| Molecular Weight(MW) | 101858 Da |
| Function | Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity (By similarity) |
| Tissue Specificity | Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung |
| Cellular Localization | Nucleus. Cytoplasm. Note=Accumulates in the major satellite repeats at pericentric heterochromatin (By similarity) |
| Entrez Gene | 1788 |
|---|---|
| Nucleotide Accession | NP_783328.1 NP_715640.2 |
Application
DNMT3A Antibody (Center R478) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the DNMT3A antibody detected the DNMT3A protein (arrow).
Application
DNMT3A Antibody (Center R478WMutant) (AP14633c)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DNMT3A Antibody (Center R478WMutant) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
