Application
DOC2A Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.Application
DOC2A Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the DOC2A antibody detected the DOC2A protein (arrow).Application
DOC2A Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DOC2A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | DOC2A Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DOC2A; Double C2-like domain-containing protein alpha |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FCWBIHC |
| FC:1:10~50 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | DOC2A Double C2-like domain-containing protein alpha |
|---|---|
| Molecular Weight(MW) | 43959 Da |
| Function | Calcium sensor which most probably regulates fusion of vesicles with membranes. Binds calcium and phospholipids. May be involved in calcium dependent neurotransmitter release through the interaction with UNC13A. May be involved in calcium-dependent spontaneous release of neurotransmitter in absence of action potentials in neuronal cells. Regulates Ca(2+)-dependent secretory lysosome exocytosis in mast cells |
| Tissue Specificity | Predominantly expressed in brain. Also expressed in testis |
| Cellular Localization | Lysosome (By similarity). Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Peripheral membrane protein (Probable). Cell junction, synapse, synaptosome Note=Colocalizes to synaptic vesicles |
| Entrez Gene | 8448 |
|---|---|
| Nucleotide Accession | NP_003577.2 |
Application
DOC2A Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Application
DOC2A Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the DOC2A antibody detected the DOC2A protein (arrow).
Application
DOC2A Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DOC2A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | FC:1:10~50 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
