Application
Western blot analysis of DOLK Antibody (Center) in 293 cell line lysates (35ug/lane). DOLK (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with DOLK Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
DOLK Antibody (Center) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | DOLK Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DOLK; KIAA1094; TMEM15; Dolichol kinase; Transmembrane protein 15 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA1094 TMEM15 |
|---|---|
| Alternative Names | DOLK KIAA1094 TMEM15 Dolichol kinase Transmembrane protein 15 |
| Molecular Weight(MW) | 59268 Da |
| Function | Involved in the synthesis of the sugar donor Dol-P-Man which is required in the synthesis of N-linked and O-linked oligosaccharides and for that of GPI anchors (By similarity) |
| Tissue Specificity | Ubiquitous. |
| Cellular Localization | Endoplasmic reticulum membrane; Multi-pass membrane protein (Potential) |
| Entrez Gene | 22845 |
|---|
Application
Western blot analysis of DOLK Antibody (Center) in 293 cell line lysates (35ug/lane). DOLK (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with DOLK Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
DOLK Antibody (Center) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
