Application
Western blot analysis of DPM1 antibody (Center) (Cat.# AP7351c) in HepG2 cell line lysates (35ug/lane). DPM1 (arrow) was detected using the purified Pab.Application
Western blot analysis of DPM1 antibody (Center) (Cat.# AP7351c) in mouse heart tissue lysates (35ug/lane). DPM1 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain tissue with DPM1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of 293 cells using DPM1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | DPM1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DPM1; Dolichol-phosphate mannosyltransferase; Dolichol-phosphate mannose synthase; Dolichyl-phosphate beta-D-mannosyltransferase; Mannose-P-dolichol synthase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | DPM1 Dolichol-phosphate mannosyltransferase Dolichol-phosphate mannose synthase Dolichyl-phosphate beta-D-mannosyltransferase Mannose-P-dolichol synthase |
|---|---|
| Molecular Weight(MW) | 29634 Da |
| Function | Transfers mannose from GDP-mannose to dolichol monophosphate to form dolichol phosphate mannose (Dol-P-Man) which is the mannosyl donor in pathways leading to N-glycosylation, glycosyl phosphatidylinositol membrane anchoring, and O- mannosylation of proteins |
| Tissue Specificity | This DPM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115~145 amino acids from the Center region of human DPM1. |
| Cellular Localization | Endoplasmic reticulum. |
| Entrez Gene | 8813 |
|---|
Application
Western blot analysis of DPM1 antibody (Center) (Cat.# AP7351c) in HepG2 cell line lysates (35ug/lane). DPM1 (arrow) was detected using the purified Pab.
Application
Western blot analysis of DPM1 antibody (Center) (Cat.# AP7351c) in mouse heart tissue lysates (35ug/lane). DPM1 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain tissue with DPM1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of 293 cells using DPM1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
