Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with *DRAM antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of anti-DRAM Antibody (N-term) in HepG2 cell line lysates (35ug/lane). DRAM (arrow) was detected using the purified Pab (1:60 dilution).| Product Name | DRAM Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DRAM1; DRAM; DNA damage-regulated autophagy modulator protein 1; Damage-regulated autophagy modulator |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| IHC:1:10~50 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | DRAM |
|---|---|
| Alternative Names | DRAM1 DRAM DNA damage-regulated autophagy modulator protein 1 Damage-regulated autophagy modulator |
| Molecular Weight(MW) | 26253 Da |
| Function | Lysosomal modulator of autophagy that plays a central role in p53/TP53-mediated apoptosis. Not involved in p73/TP73- mediated autophagy |
| Tissue Specificity | This DRAM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human DRAM. |
| Cellular Localization | Lysosome membrane; Multi-pass membrane protein |
| Entrez Gene | 55332 |
|---|
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with *DRAM antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of anti-DRAM Antibody (N-term) in HepG2 cell line lysates (35ug/lane). DRAM (arrow) was detected using the purified Pab (1:60 dilution).| Application Notes | IHC:1:10~50 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein A affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
Data sheet for OM231585