Application
Immunohistochemistry of DRAM in human liver tissue with DRAM antibody at 2.5 μg/mL.Application
Immunofluorescence of DRAM in Human Liver tissue with DRAM antibody at 20 μg/mL.Application
Western blot analysis of DRAM in K562 cell lysate with DRAM antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.| Product Name | DRAM Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DRAM Antibody: DRAM, DRAM, DNA damage-regulated autophagy modulator protein 1, Damage-regulated autophagy modulator |
| Product description | DRAM Antibody: Damage-regulated autophagy modulator (DRAM) is a p53 target gene encoding a lysosomal protein that induces autophagy, a process that degrades cytosolic proteins and organelles. It has been suggested that activation of DRAM by p53 is simultaneous to the activation by p53 of one or more proapoptotic genes such as PUMA, Bax, etc., and that the signaling pathways regulated by these genes together promote a full cell death response. By itself, DRAM cannot induce apoptosis, but the fact that it is inactivated in certain cancers highlights the importance of DRAM and suggests that autophagy may play a more important role in cancer than initially suspected. At least two different isoforms of DRAM are known to exist.1) Crighton D, Wilkinson S, O’Prey J, et al. DRAM, a p53-induced modulator of autophagy, is critical for apoptosis. Cell 2006; 126:121-34. |
| Immunogen | DRAM antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human DRAM. The immunogen is located within amino acids 30 - 80 of DRAM. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| DRAM antibody can be used for detection of DRAM by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DRAM |
|---|---|
| Alternative Names | DRAM Antibody: DRAM DRAM DNA damage-regulated autophagy modulator protein 1 Damage-regulated autophagy modulator |
| Molecular Weight(MW) | Predicted: 26 kDa Observed: 30 kDa |
| Sequence Similarities | 3) Cat. No. 10-201 - Human Liver Tissue Slide |
Application
Immunohistochemistry of DRAM in human liver tissue with DRAM antibody at 2.5 μg/mL.
Application
Immunofluorescence of DRAM in Human Liver tissue with DRAM antibody at 20 μg/mL.
Application
Western blot analysis of DRAM in K562 cell lysate with DRAM antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | DRAM antibody can be used for detection of DRAM by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | DRAM antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | DRAM Antibody is supplied in PBS containing 0.02% sodium azide. |
Data sheet for OM277366