Application
Western blot analysis of DTX2 Antibody (C-term) in MDA-MB435, MDA-MB231 cell line lysates (35ug/lane). DTX2 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with DTX2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | DTX2 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DTX2; KIAA1528; RNF58; Probable E3 ubiquitin-protein ligase DTX2; Protein deltex-2; RING finger protein 58 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA1528 RNF58 |
|---|---|
| Alternative Names | DTX2 KIAA1528 RNF58 Probable E3 ubiquitin-protein ligase DTX2 Protein deltex-2 RING finger protein 58 |
| Molecular Weight(MW) | 67246 Da |
| Function | Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Probably acts both as a positive and negative regulator of Notch, depending on the developmental and cell context. Mediates the antineural activity of Notch, possibly by inhibiting the transcriptional activation mediated by MATCH1. Functions as an ubiquitin ligase protein in vitro, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity |
| Tissue Specificity | This DTX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 579~609 amino acids from the C-terminal region of human DTX2. |
| Cellular Localization | Cytoplasm. Nucleus. Note=Predominantly cytoplasmic. Partially nuclear |
| Entrez Gene | 113878 |
|---|
Application
Western blot analysis of DTX2 Antibody (C-term) in MDA-MB435, MDA-MB231 cell line lysates (35ug/lane). DTX2 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with DTX2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
