Application
Western blot analysis of DTX4 Antibody (N-term) in HL-60 cell line lysates (35ug/lane). DTX4 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with DTX4 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
DTX4 Antibody (N-term) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | DTX4 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DTX4; KIAA0937; RNF155; E3 ubiquitin-protein ligase DTX4; Protein deltex-4; RING finger protein 155 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100-500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0937 RNF155 |
|---|---|
| Alternative Names | DTX4 KIAA0937 RNF155 E3 ubiquitin-protein ligase DTX4 Protein deltex-4 RING finger protein 155 |
| Molecular Weight(MW) | 67258 Da |
| Function | Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations (By similarity). Functions as an ubiquitin ligase protein in vivo, mediating 'Lys48'-linked polyubiquitination and promoting degradation of TBK1, targeting to TBK1 requires interaction with NLRP4 |
| Tissue Specificity | This DTX4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62~92 amino acids from the N-terminal region of human DTX4. |
| Cellular Localization | Cytoplasm (By similarity). |
| Entrez Gene | 23220 |
|---|
Application
Western blot analysis of DTX4 Antibody (N-term) in HL-60 cell line lysates (35ug/lane). DTX4 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with DTX4 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
DTX4 Antibody (N-term) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100-500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
