Application
Western blot analysis of E1 Ubiquitin (UBE1) Antibody (C-term) in mouse stomach tissue lysates (35ug/lane). UBE1 (arrow) was detected using the purified Pab.Application
The anti-UBE1 Pab is used in Western blot to detect UBE1 in HL-60 cell lysate.| Product Name | E1 Ubiquitin (UBE1) Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UBA1; A1S9T; UBE1; Ubiquitin-like modifier-activating enzyme 1; Protein A1S9; Ubiquitin-activating enzyme E1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | A1S9T UBE1 |
|---|---|
| Alternative Names | UBA1 A1S9T UBE1 Ubiquitin-like modifier-activating enzyme 1 Protein A1S9 Ubiquitin-activating enzyme E1 |
| Molecular Weight(MW) | 117849 Da |
| Function | Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin- E1 thioester and free AMP |
| Tissue Specificity | This UBE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human UBE1. |
| Entrez Gene | 7317 |
|---|
Application
Western blot analysis of E1 Ubiquitin (UBE1) Antibody (C-term) in mouse stomach tissue lysates (35ug/lane). UBE1 (arrow) was detected using the purified Pab.
Application
The anti-UBE1 Pab is used in Western blot to detect UBE1 in HL-60 cell lysate.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
