Application
The anti-E2EPF Pab is used in Western blot to detect E2EPF in HL-60 cell lysate.Application
Western blot analysis of E2EPF (arrow) using E2EPF Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the UBE2S gene (Lane 2) (Origene Technologies).Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | E2EPF Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UBE2S; E2EPF; Ubiquitin-conjugating enzyme E2 S; E2-EPF; Ubiquitin carrier protein S; Ubiquitin-conjugating enzyme E2-24 kDa; Ubiquitin-conjugating enzyme E2-EPF5; Ubiquitin-protein ligase S |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | E2EPF |
|---|---|
| Alternative Names | UBE2S E2EPF Ubiquitin-conjugating enzyme E2 S E2-EPF Ubiquitin carrier protein S Ubiquitin-conjugating enzyme E2-24 kDa Ubiquitin-conjugating enzyme E2-EPF5 Ubiquitin-protein ligase S |
| Molecular Weight(MW) | 23845 Da |
| Function | Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as a E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except 'Lys-48'-linked polyubiquitination |
| Tissue Specificity | This E2EPF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 18~47 amino acids from the N-terminal region of human E2EPF. |
| Entrez Gene | 27338 |
|---|
Application
The anti-E2EPF Pab is used in Western blot to detect E2EPF in HL-60 cell lysate.
Application
Western blot analysis of E2EPF (arrow) using E2EPF Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the UBE2S gene (Lane 2) (Origene Technologies).
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
