Application
Western blot analysis of anti-EARS2 Pab in HL60 cell line lysates (35ug/lane).EARS2 (arrow) was detected using the purified Pab (1:60 dilution).Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with EARS2 antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | EARS2 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EARS2; KIAA1970; Probable glutamate--tRNA ligase, mitochondrial; Glutamyl-tRNA synthetase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA1970 |
|---|---|
| Alternative Names | EARS2 KIAA1970 Probable glutamate--tRNA ligase mitochondrial Glutamyl-tRNA synthetase |
| Molecular Weight(MW) | 58689 Da |
| Function | Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) (By similarity) |
| Tissue Specificity | This EARS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 494~523 amino acids from the C-terminal region of human EARS2. |
| Cellular Localization | Mitochondrion matrix (By similarity). |
| Entrez Gene | 124454 |
|---|
Application
Western blot analysis of anti-EARS2 Pab in HL60 cell line lysates (35ug/lane).EARS2 (arrow) was detected using the purified Pab (1:60 dilution).
Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with EARS2 antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
